如何Productive地生产一批感受态细胞 How to Make Chemically Competent E. coli
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「Protocols」大肠杆菌感受态细胞制作的Standard Procedure Preparation of Competent E. Coli Cells
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如何高效率进行大肠杆菌的电转化实验 Chemical Transformation of E. coli
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如何设计gRNA用于CRISPR敲除系统。Designing gRNA Oligos
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反向PCR（ Inverse PCR） 的原理及使用场景Part1 Inverse PCR Explained
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如何精准地利用大肠杆菌将某蛋白表达纯化 Bacterial Protein Expression & Purification
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细胞基因编辑技术：gRNA、PAM、Cas9 #3
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如何有效测定辣根过氧化物酶酶动力学 Enzyme Kinetics of Horseradish Peroxidase
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抗生素作用机制和耐药性的产生 Mechanisms of Action and Antibiotic Resistance
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通过Heat Shock完美滴将质粒转化到DH5a中得到Beautiful的克隆子 How to Transform E. coli by Heat Shock
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「Method」Gateway克隆 Gateway Cloning
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如何高效地从哺乳动物细胞中分离目的蛋白 Protein Isolation from Mammalian Cells
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【Knowledge】今天来聊聊噬菌体展示技术 Phage Display Technology (Updated Version)
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「Protocols」细菌怎么养才给力？液体细菌培养的方法，抗生素浓度的选择等 Inoculating Liquid Bacterial Culture
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有关分子实验的一些基础技术 PCR, Restriction Digestion, Agarose Gel Electrophoresis
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「SnapGene」用SnapGene模拟琼脂糖凝胶电泳 Simulating Agarose Gel Electrophoresis In SnapGene
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用单核苷酸多态性预测味觉能力Part2 Single Nucleotide Polymorphism to Predict Bitter Taste
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如何用聚丙烯酰胺凝胶电泳跑出犀利的蛋白胶 SDS-PAGE
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「Knowledge」什么是细胞转染？有哪些方法。What is transfection - Polyplus transfection
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如何以一个Beautiful的比例进行DNA片段的连接 Ligation with T4 DNA Ligase
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如何precisely地解释通过热激转化Plasmid的原理 Heat shockThe Mechanism of Transformation
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「SnapGene」用SnapGene模拟限制性酶切和DNA片段插入 Simulating Restriction And Insertion Cloning
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细胞基因编辑技术：gRNA、PAM、Cas9
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「SnapGene」用SnapGene对质粒进行特征分析 Introduction to Visualization Features in SnapGene
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如何用PCR的手段Select正确的克隆 How to Screen Bacterial Colonies with PCR
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桑格测序的样品准备和数据分析 Sanger Sequencing - Sample Prep & Data Analysis with BLAST
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Bacterial transformation - chemically competent cells & heat shock
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「MEGA X」进化树的创建和优化 MEGA X Reconstructing Ancestral Sequences
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「Method」如何用Excel计算 皮尔逊相关检验 How To Perform A Pearson Correlation Test In Excel
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「GraphPad」GraphPad小提琴图的绘制及注释 How To Create A Violin Plot in GraphPad Prism
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「用结晶紫检测细胞毒性」Assessing cytotoxicity using crystal violet
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「Snapgene」用SnapGene模拟Gateway克隆 Simulating Gateway Cloning In SnapGene
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「Protocol」质粒DNA转染细胞实验 Plasmid DNA Transfection Protocol
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「SnapGene」如何给感兴趣的基因作注释 Working With Features In SnapGene
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「SnapGene」用SnapGene模拟无缝克隆 Simulating In-Fusion Cloning In SnapGene
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「GraphPad」如何用GraphPad画出理想的散点图 How To Create A Scatter Plot In GraphPad Prism
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「Equipment」Old antique！实验室电穿孔仪 Laboratory Electroporator
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「SnapGene」测序回来的质粒怎么进行下一步分析？
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北京周口店猿人山顶洞人与我们有什么关系？
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「MEGA X」将获取的DNA序列或protein序列保存 MEGA X Choosing and Acquiring Sequences Part 2
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阿三教你——DNA复制—延伸！DNA Replication in Eukaryotes | Elongation