V
主页
京.东618红包,每天可领3次
8. 同源重组
发布人
An essential requirement of DNA replication is to maintain the integrity of the genetic material. For this reason double-strand breaks are preferentially repaired by homologous recombination and it is typically carried out after DNA synthesis when the two daughter DNA molecules are in proximity, and one can serve as a template for repair of the other. In eukaryotes, a protein complex called MRN, composed of specialized nucleases degrade the damaged ends of the DNA while ends tethered to each other. The DNA is left with single-strand overhangs of 3-4 nucleotides with a 3’ OH end. This single-stranded section is stabilized by RPA proteins until Rad51 - homolog of prokaryotic protein RecA - is activated by ATP, and binds to the DNA forming a filament. In the filament, the DNA exists as triplets of nucleotides where the DNA backbone is unwound between adjacent triplets. This DNA-protein filament binds to a duplex DNA from a sister chromatid by stretching the intact template DNA and destabilizing it, so that the two strands can be easily pulled apart and the broken strand can attempt to bind to the template, in a process known as strand invasion. The invading strand searches for undamaged, homologous sequences in the genomic DNA by trying to form base pairs in a block of three nucleotides. If the basepairs mismatch, the invading DNA dissociates and looks for other homologous regions. If one triplet from the invading strand matches with the template, then the next three nucleotides are sampled. Following this, a helicase displaces the now extended invading strand which basepairs with the uncoated damaged strand. Next, the second damaged strand anneals to the complementary strand of the template DNA for another round of DNA synthesis. Finally, the sister strands dissociate and a DNA ligase seals the nicks, restoring the repaired helices, and ensuring accurate repair of the intact chromosome.
打开封面
下载高清视频
观看高清视频
视频下载器
【中英】同源染色体重组 Holliday模型-细胞生物学动画
同源重组(Holliday模型)-homologous recombination-动画
通过同源重组的原理敲除细菌基因-第1集
一种基因敲除方法:同源重组双交换
引物设计-同源重组方法,有问题欢迎探讨
同源重组 链接产物转化大肠杆菌流程
华中科技大学蒋涛老师 分子生物学 同源重组 CSSR 20231019_075814
基因重组--同源重组 homologous recombination
DNA损伤和修复( DNA Damage and Repair)
同源重组的分子机制
【短视频大赛】使用snapgene软件的infusion功能实现载体的同源重组
同源重组法-引物在线设计
「NHEJ」非同源末端连接|DNA修复原理
snapgene构建同源重组质粒
通过同源重组的原理敲除细菌基因-第2集
【生化】DNA重组和重组DNA技术【温医大】
02.DNA损伤修复(直接修复、切除修复、碱基错配修复、重组修复)
【生物问题解答】1 读书方法 同源重组
03上下游同源臂的确定
了解同源重组修复缺陷(HRD)及其检测方法
2.基础切除修复
【DNA双链断裂|同源重组修复】Homologous Recombination for Double Strand Breaks Part 2
分子生物学实验-快速载体构建之同源重组技术ClonExpress
第29讲 DNA的复制损伤修复及重组四
DNA的复制过程-3D动画
基因工程题(同源重组)
#载体构建基础-2#Gateway,无缝克隆(同源重组)
DNA重组质粒的构建
red/ET同源重组技术构建质粒(模拟且粗略的流程)
转座重组-transposition-动画
非同源末端重组演示
Snapgene分子克隆之重组质粒的构建2.无缝克隆(引物设计同源臂、反向PCR)
DNA Repair & Recombination _ Cell Biology DNA修复与重组_细胞生物学
【无缝克隆/同源重组】无缝克隆引物设计
基因组稳定性与DNA损伤修复(碱基损伤、突变、切除修复、重组修复)【分子生物学期末速成】
唯赞学堂 | 同源重组克隆原理及解决方案
同源定向修复HDR简介及CRISPR knock-in原理
DNA修复和重组
【分子复盘】DNA复制修复+DNA重组
0基础CRISPR-Cas9原理介绍